Early Virus–Host Cell Interactions

Early Bunyavirus-Host Cell Interactions

The Bunyaviridae is the largest family of RNA viruses, with over 350 members worldwide. Several of these viruses cause severe diseases in livestock and humans. With an increasing number and frequency of outbreaks, bunyaviruses represent a growing threat to public health and agricultural productivity globally. Yet, the receptors, cellular factors and endocytic pathways used by these emerging pat...

Early events in cell-animal virus interactions.

Direct T helper-B cell interactions induce an early B cell activation antigen

We have explored the consequences for the B cell of cognate interaction with T cells. Early expression of the B cell-restricted cell surface activation antigen, BLAST-2, has been used as an assay system to measure direct T-B cell collaboration. BLAST-2 is preferentially expressed by allogenic B cells cultured with MHC class II antigen-restricted Th clone cells matched to the DR specificity of t...

Dynamic migration and cell-cell interactions of early reprogramming revealed by high-resolution time-lapse imaging.

Discovery of the cellular and molecular mechanisms of induced pluripotency has been hampered by its low efficiency and slow kinetics. Here, we report an experimental system with multicolor time-lapse microscopy that permits direct observation of pluripotency induction at single cell resolution, with temporal intervals as short as 5 minutes. Using granulocyte-monocyte progenitors as source cells...

Cell–cell and cell–matrix interactions

Roles of cell protrusions as initial and dynamic mediators of cell–cell and cell–ECM interactions Filopodia have well-established roles in sampling the local environment and making initial cell–ECM contacts at the leading edge of many cell types. Filopodial-like structures, termed “epithelial zippers,” can mediate initial contacts between epithelial cells that culminate in assembly of cell–cell...